Gene Editing Institute at Christiana Care Health System’s Helen F. Graham Cancer Center & Research Institute, works directly in the design, optimization and application of CRISPR gene editing tools that can be used across a wide array of biomedical researches. With affordability in mind, the Institute provides customized CRISPR services with improved and streamlined CRISPR/Cas9 processes that allow timely delivery of high-quality clonal cell lines with knock-out or various knock-in mutations.

Genome Customization Timeline

Service Offerings:

Knock-out services:

  • Single gene knockout clonal cell lines
  • Multiplex knockout clonal cell lines
  • Large deletion clonal cell lines
  • Knockout pools

Knock-in services:

  • Point-mutation clonal cell lines
  • Insertional mutation (epitope tagging, etc) clonal cell lines
  • Large insertional mutation (fluorescent protein fusion, transcriptional termination, etc) clonal cell lines
  • Multiplex knock-in clonal cell lines

Knock-out library services:

  • Whole human genome knock-out libraries (6 gRNAs per gene)
  • Targeted genome knock-out libraries (6 gRNAs per gene)

Specialized services:

  • Gene repression through CRISPR/Cas9i
  • Gene activation through CRISPR/Cas9a

Turnaround Time (wks)

  Easy Cell Line1** Difficult Cell Line2**
Knock-Out 10±2 12±4
Knock-In, Epitope Tagging
(HA, FLAG, MYC, etc.)
12±2 14±4
Knock-In, Mutation
Point mutation
12±2 14±4
Knock-In, Long
Fluorescent protein tagging or other specialized insertions
14±2 16±4

  1. Easy cell lines: uncomplicated transfectability and clonality. Examples include A549, HeLa, HCT116, THP-1, HEK293, MIN6, PC3, HepG2, etc.
  2. Difficult cell lines: complicated transfectability and clonality, requiring extra process and project time. Examples include LNCap, VCap, 92-1, etc.

**Estimated turnaround time

Deliverables:

Knock-out:

  • 2 KO cell clones
  • Unmodified parental cells
  • Milestone reports
  • Sequence analysis
  • Certificate of analysis

Knock-in:

  • 2 KI cell clones (knock-in in one or more alleles)
  • Unmodified parental cells
  • Milestone reports
  • Sequence analysis
  • Certificate of analysis

For more information, call 302-623-4761 or
e-mail:
geneeditinginstitute@christianacare.org