Gene Editing Institute at Christiana Care Health System’s Helen F. Graham Cancer Center & Research Institute, works directly in the design, optimization and application of CRISPR gene editing tools that can be used across a wide array of biomedical researches. With affordability in mind, the Institute provides customized CRISPR services with improved and streamlined CRISPR/Cas9 processes that allow timely delivery of high-quality clonal cell lines with knock-out or various knock-in mutations.
Service Offerings:
Knock-out services:
- Single gene knockout clonal cell lines
- Multiplex knockout clonal cell lines
- Large deletion clonal cell lines
- Knockout pools
Knock-in services:
- Point-mutation clonal cell lines
- Insertional mutation (epitope tagging, etc) clonal cell lines
- Large insertional mutation (fluorescent protein fusion, transcriptional termination, etc) clonal cell lines
- Multiplex knock-in clonal cell lines
Knock-out library services:
- Whole human genome knock-out libraries (6 gRNAs per gene)
- Targeted genome knock-out libraries (6 gRNAs per gene)
Specialized services:
- Gene repression through CRISPR/Cas9i
- Gene activation through CRISPR/Cas9a
Turnaround Time (wks)
| Easy Cell Line1** | Difficult Cell Line2** | |
|---|---|---|
| Knock-Out | 10±2 | 12±4 |
| Knock-In, Epitope Tagging (HA, FLAG, MYC, etc.) |
12±2 | 14±4 |
| Knock-In, Mutation Point mutation |
12±2 | 14±4 |
| Knock-In, Long Fluorescent protein tagging or other specialized insertions |
14±2 | 16±4 |
- Easy cell lines: uncomplicated transfectability and clonality. Examples include A549, HeLa, HCT116, THP-1, HEK293, MIN6, PC3, HepG2, etc.
- Difficult cell lines: complicated transfectability and clonality, requiring extra process and project time. Examples include LNCap, VCap, 92-1, etc.
**Estimated turnaround time
Deliverables:
Knock-out:
- 2 KO cell clones
- Unmodified parental cells
- Milestone reports
- Sequence analysis
- Certificate of analysis
Knock-in:
- 2 KI cell clones (knock-in in one or more alleles)
- Unmodified parental cells
- Milestone reports
- Sequence analysis
- Certificate of analysis