Perception, intention, and action in adolescent obesity

Written by Bittner Fagan H, Diamond J, Myers R, Gill JMon

Bittner Fagan H, Diamond J, Myers R, Gill JM J Am Board Fam Med 2008 Nov-Dec;21(6):555-61 PMID: 18988723 Abstract BACKGROUND: Insight into adolescents’ weight-loss behavior is needed. METHODS: Survey data were obtained from overweight and obese adolescents in the Youth Risk Behavioral Survey (YRBS) in Delaware. Cross tabulations were used to determine the frequency of

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Tailored navigation in colorectal cancer screening

Written by Myers RE, Hyslop T, Sifri R, Bittner-Fagan H, Katurakes NC, Cocroft J, Dicarlo M, Wolf Ton

Myers RE, Hyslop T, Sifri R, Bittner-Fagan H, Katurakes NC, Cocroft J, Dicarlo M, Wolf T Med Care 2008 Sep;46(9 Suppl 1):S123-31 PMID: 18725824 Abstract BACKGROUND: Colorectal cancer (CRC) screening is underutilized. Effective methods to increase screening use are needed. This study sought to determine the impact of tailored navigation on CRC screening in primary

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DNA replication, cell cycle progression and the targeted gene repair reaction

Written by Engstrom JU, Kmiec EBon

Engstrom JU, Kmiec EB Cell Cycle 2008 May;7(10):1402-14 PMID: 18424915 Abstract Single-stranded oligonucleotides (ssODNs) can direct base changes in mammalian cells and influence changes in phenotype. The mechanism by which ssODNs alters the sequence is being revealed by studies carried out in model systems. In the long run, this information will provide the basis for

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Targeted gene repair activates Chk1 and Chk2 and stalls replication in corrected cells

Written by Ferrara L, Kmiec EBon

Ferrara L, Kmiec EB DNA Repair (Amst.) 2006 Apr;5(4):422-31 PMID: 16414312 Abstract Oligonucleotides (ODNs) can direct the exchange of single nucleotides at specific sites in the mammalian genome. It is generally believed that the ODN aligns in homologous register with its complementary site in the target gene and provides a template for the endogenous repair

Continue reading Targeted gene repair activates Chk1 and Chk2 and stalls replication in corrected cells

Gene repair in mammalian cells is stimulated by the elongation of S phase and transient stalling of replication forks

Written by Brachman EE, Kmiec EBon

Brachman EE, Kmiec EB DNA Repair (Amst.) 2005 Apr;4(4):445-57 PMID: 15725625 Abstract The repair of point mutations directed by modified single-stranded DNA oligonucleotides is dependent on the activity of proteins involved in homologous recombination (HR). As a consequence, factors that stimulate homologous recombination, such as double strand breaks, can impact the frequency with which repair

Continue reading Gene repair in mammalian cells is stimulated by the elongation of S phase and transient stalling of replication forks

Camptothecin enhances the frequency of oligonucleotide-directed gene repair in mammalian cells by inducing DNA damage and activating homologous recombination

Written by Ferrara L, Kmiec EBon

Ferrara L, Kmiec EB Nucleic Acids Res. 2004;32(17):5239-48 PMID: 15466591 Abstract Camptothecin (CPT) is an anticancer drug that promotes DNA breakage at replication forks and the formation of lesions that activate the processes of homologous recombination (HR) and nonhomologous end joining. We have taken advantage of the CPT-induced damage response by coupling it to gene

Continue reading Camptothecin enhances the frequency of oligonucleotide-directed gene repair in mammalian cells by inducing DNA damage and activating homologous recombination