Targeted nucleotide repair of cyc1 mutations in Saccharomyces cerevisiae directed by modified single-stranded DNA oligonucleotides

Brachman EE, Kmiec EB Genetics 2003 Feb;163(2):527-38 PMID: 12618392 Abstract Modified single-stranded DNA oligonucleotides have been used to direct base changes in the CYC1 gene of Saccharomyces cerevisiae. In this process, the oligonucleotide is believed to hybridize to the target site through the action of a DNA recombinase and, once bound, DNA repair enzymes act

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The development and regulation of gene repair

Liu L, Parekh-Olmedo H, Kmiec EB Nat. Rev. Genet. 2003 Sep;4(9):679-89 PMID: 12951569 Abstract A technique that can direct the repair of a genetic mutation in a human chromosome using the DNA repair machinery of the cell is under development. Although this approach is not as mature as other forms of gene therapy and fundamental

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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Rivera-Torres N, Kmiec EB J Vis Exp 2017 Aug;(126) PMID: 28872131 Abstract Combinatorial gene editing using CRISPR/Cas9 and single-stranded oligonucleotides is an effective strategy for the correction of single-base point mutations, which often are responsible for a variety of human inherited disorders. Using a well-established cell-based model system, the point mutation of a single-copy mutant

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CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome

Bloh KM, Bialk PA, Gopalakrishnapillai A, Kolb EA, Kmiec EB Mol Ther Nucleic Acids 2017 Jun;7:288-298 PMID: 28624204 Abstract Using a CRISPR/Cas9 system, we have reengineered a translational start site in the GATA1 gene in K562 cells. This mutation accounts largely for the onset of myeloid leukemia in Down syndrome (ML-DS). For this reengineering, we

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Importance of engaging obstetrician/gynecologists in cardiovascular disease prevention

Ehrenthal DB, Catov JM Curr. Opin. Cardiol. 2013 Sep;28(5):547-53 PMID: 23928919 Abstract PURPOSE OF REVIEW: To review recent evidence regarding traditional and sex-specific factors identified among women during their reproductive years and their importance in lifetime risk for cardiovascular disease (CVD). RECENT FINDINGS: Longitudinal studies demonstrated a woman’s burden of risk during her reproductive years

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The people in your neighborhood: impact of physician cohorting on nursing units

Lyons D, Painter J, Crannell C, Mooney R, Bucher L, Joseph R, Schwandt C, Siano S, Marandola B, Keiser S, Bayne L Medsurg Nurs 2013 Sep-Oct;22(5):281-9, 302 PMID: 24358568 Abstract In a study exploring the impact of a new physician practice model on staff’s perceptions of their work environment, no statistically significant differences were found;

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Variation in NICU Admission Rates Without Identifiable Cause

Ziegler KA, Paul DA, Hoffman M, Locke R Hosp Pediatr 2016 May;6(5):255-60 PMID: 27117951 Abstract OBJECTIVES: Admission to the NICU is influenced by physiologic compromise and by hospital care protocols. Providing appropriate care must be balanced with adverse consequences of NICU admission, such as interrupting maternal-infant bonding and unnecessary interventions. This study aims to determine

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Determining the magnitude of surveillance bias in the assessment of lower extremity deep venous thrombosis: A prospective observational study of two centers

Shackford SR, Cipolle MD, Badiee J, Mosby DL, Knudson MM, Lewis PR, McDonald VS, Olson EJ, Thompson KA, Van Gent JM, Zander AL J Trauma Acute Care Surg 2016 May;80(5):734-9; discussion 740-1 PMID: 26891155 Abstract BACKGROUND: Venous thromboembolism (VTE) remains a significant cause of morbidity and mortality in trauma. Controversy exists regarding the use of

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Improving Infusion Pump Safety Through Usability Testing

Miller KE, Arnold R, Capan M, Campbell M, Zern SC, Dressler R, Duru OO, Ebbert G, Jackson E, Learish J, Strauss D, Wu P, Bennett DA J Nurs Care Qual 2017 Apr/Jun;32(2):141-149 PMID: 27500697 Abstract With the recognition that the introduction of new technology causes changes in workflow and may introduce new errors to the

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Predicting violent behavior in clinical settings: a case-control study of a mental health inpatient unit

Miller KE, Benden ME, Shipp EM, Pickens AW, Wendel ML, Pronovost PJ, Watts BV J Healthc Prot Manage 2016;32(1):106-19 PMID: 26978965 Abstract In order to assist staff in recognizing patients prone to violence and guide their clinical decision-making, this study summarizes mental health inpatient unit incidents over a one-year period. Results describe demographic and clinical

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